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Sarkytob — Stem cross-section grape berry

Published: 2011-09-27 18:12:40 +0000 UTC; Views: 2824; Favourites: 35; Downloads: 88
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Here you can see a micrography of a cross-section through the pedicel of a grape berry (the part connecting the single fruit to the bunch ) at 20-fold magnification.
It was stained to contrast the xylem-vessels (the tubes for long-distance water transport in a plant) - the red to pink part of the picture.
It consist of many single images, each showing another sector of the cross-section. It was taken with a motorized micoscop stage which automatically shiftet the slide containing the cross-section to the next section after taking a photo of the current section in view. So it was scanned piecewise.
The original image had 5201x5498 pixel at 200dpi, i reduced both to make it more handsome for the upload (as uncompressed tif it had roundabout 84 MB).

I just like the outcome (Even so the cross-section wasn't done optimal so it was unequally thick which lead to some out-off-focus parts)
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Comments: 59

Else22 In reply to ??? [2011-10-03 11:37:50 +0000 UTC]

I suppose the centre is coloured so as to be distinguished from the rest of it, right? But how did you manage to colour it?

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Sarkytob In reply to Else22 [2011-10-03 12:31:53 +0000 UTC]

Yeah right. The centre is coloured with a stain called phloroglucin and hydrochloric acid. This reagent stains specifically lignin, a main component of "wood". Wood on the other hand is builded mainly in perennial organs, so fruits and leaves wich are normally annual don't contain many woody parts. A exclusion are the vessels for water transport which have to stand high pressures and therefore are strengthened by lignin. So it was only a question of choosing a specific staining agent

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Else22 In reply to Sarkytob [2011-10-03 18:01:39 +0000 UTC]

I understand Thank you for the extra-info

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Sarkytob In reply to Else22 [2011-10-03 18:38:34 +0000 UTC]

No problem, thanks for your interest

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nathalie-baugi In reply to ??? [2011-09-27 21:51:51 +0000 UTC]

definitely true, this deserves to get daily deviation-ed

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Sarkytob In reply to nathalie-baugi [2011-09-27 22:20:34 +0000 UTC]

Wuhuu, ty again, i'm flattered and cajoled

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nathalie-baugi In reply to ??? [2011-09-27 18:14:47 +0000 UTC]

amazing

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Sarkytob In reply to nathalie-baugi [2011-09-27 21:49:53 +0000 UTC]

Thanks a lot, i used this picture actually for some measurments and decided afterwards it would be a pity if it will be only present on my harddisk

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