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Description
Ashi from Samurai Jack
I know it's not Samurai Jack version created
Ashi was born by the hands of High Priestess

Ashi was the deuteragonist (though in the series as a whole, Aku could fill this role, while Ashi shares the role of tritagonist with the Scotsman and the Emperor . She was also originally quaternary antagonist before becoming an ally of Samurai Jack in Season 5 ) of Samurai Jack . She was the only confirmed living member of the Daughters of Aku , seven sisters who served as assassins attempting to kill Jack . It is only later that she came to have doubts about Aku's true nature after Jack saves her life and she makes a full turn-around when Jack shows her the destruction Aku has unleashed on the world.
She served as Jack's most important ally in Season 5, in Episode XCIX she and Jack eventually fall in love with each other. Eventually, after meeting Aku in person in Episode C , it is revealed that Aku was her biological father, after which he corrupts her unwillingly into his service. In Episode CI , she used her powers as a human-demon hybrid to help transport Jack back to the past to defeat Aku, but tragically passes away on the day of her wedding to Jack as a consequence of past Aku's death.

In the Network League timeline, Ashi joins with Samurai Jack after Jack convince him and do it against her mother
She dies in invasion in Network League: Unlimited (Season 2) but Alternate Ashi gave her soul away to Ashi who came back from the grave and return to life to be with Jack

Edit: New Version

Samurai Jack (c) Genndy Tartakovsky

Description
Ashi from Samurai Jack
I know it's not Samurai Jack version created
Ashi was born by the hands of High Priestess

Ashi was the deuteragonist (though in the series as a whole, Aku could fill this role, while Ashi shares the role of tritagonist with the Scotsman and the Emperor . She was also originally quaternary antagonist before becoming an ally of Samurai Jack in Season 5 ) of Samurai Jack . She was the only confirmed living member of the Daughters of Aku , seven sisters who served as assassins attempting to kill Jack . It is only later that she came to have doubts about Aku's true nature after Jack saves her life and she makes a full turn-around when Jack shows her the destruction Aku has unleashed on the world.
She served as Jack's most important ally in Season 5, in Episode XCIX she and Jack eventually fall in love with each other. Eventually, after meeting Aku in person in Episode C , it is revealed that Aku was her biological father, after which he corrupts her unwillingly into his service. In Episode CI , she used her powers as a human-demon hybrid to help transport Jack back to the past to defeat Aku, but tragically passes away on the day of her wedding to Jack as a consequence of past Aku's death.

In the Network League timeline, Ashi joins with Samurai Jack after Jack convince him and do it against her mother
She dies in invasion in Network League: Unlimited (Season 2) but Alternate Ashi gave her soul away to Ashi who came back from the grave and return to life to be with Jack

Edit: New Version

Samurai Jack (c) Genndy Tartakovsky