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Published: 2018-03-10 23:14:34 +0000 UTC; Views: 2207; Favourites: 4; Downloads: 6
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Description You will probably ask me what the hell that is and it is a fair question. I will probably move this to scraps soon, but I wanted to share some schemes I did for my poster last year.

This is a two-electrode voltage clamp setup that allows to record ion channel activity in real time. The brown and white ball is actually a frog oocyte that you can make express any protein you want and it is much simpler than to record channels in their native environment (like in the neurons) as Xenopus oocytes are just huge (over 1 mm in diameter)! Since the 1980s many ion channels have been characterized by expression in Xenopus oocytes, including many that, when mutated, cause serious human disease.

I tried to make this scheme aesthetically appealing, but it is one of my first tries with vector graphics, so let me know what you think of it!
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Comments: 5

gnomishblend [2018-04-22 18:51:24 +0000 UTC]

Not so basic textbook biology. Great work!

👍: 0 ⏩: 1

RiEile In reply to gnomishblend [2018-04-23 10:37:10 +0000 UTC]

Back to the classical methods. Thank you for commenting

👍: 0 ⏩: 0

Cake-a-Tron [2018-03-11 01:23:20 +0000 UTC]

Well it looks pretty darn neat, that's for sure! It all sounds really complicated but the setup is relatively easy to follow. 

👍: 0 ⏩: 1

RiEile In reply to Cake-a-Tron [2018-03-11 16:25:11 +0000 UTC]

Thank you Old electrophysiology setups are quite simple indeed.

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Cake-a-Tron In reply to RiEile [2018-03-12 01:28:37 +0000 UTC]

You're welcome! Maybe so, but they sure don't sound simple X)

👍: 0 ⏩: 0